Ascorbic acid determination with an automated enzymatic procedure
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چکیده
منابع مشابه
Ascorbic acid determination with an automated enzymatic procedure.
Given the widespread interest in antioxidant nutrients, we have developed a new procedure that will permit the automated determination of plasma ascorbic acid (AA) concentration with a Roche Fara centrifugal analyzer. After the deproteinization of plasma with metaphosphoric acid, AA is oxidized to dehydroascorbic acid by AA oxidase. The product is coupled to o-phenylenediamine to produce a chro...
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‘J’HE MOST COMMONLY USED METHODS for the estimation of uric acid are those colorimetric procedures utilizing the reduction of phosphotungstic acid (1-7). Since these procedures are not specific for uric acid, an enzymatic procedure utilizing uricase, which oxidizes uric acid to allantoin, has been employed by a number of investigators (8-12). The enzymatic method, dependent upon the absorption ...
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 1997
ISSN: 0009-9147,1530-8561
DOI: 10.1093/clinchem/43.1.154